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Olive flounder (Paralichthys olivaceus) muscle satellite cells (OFMCs) were obtained by enzymatic primary cell isolation and the explant method. Enzymatic isolation yielded cells that reached 80% confluence within 8 days, compared to 15 days for the explant method. Optimal OFMC growth was observed in 20% fetal bovine serum at 28 °C with 0.8 mM CaCl2 and the basic fibroblast growth factor (BFGF) to enhance cell growth. OFMCs have become permanent cell lines through the spontaneous immortalization crisis at the 20th passage. Olive flounder skeletal muscle myoblasts were induced into a mitogen-poor medium containing 2% horse serum for differentiation; they fused to form multinucleate myotubes. The results indicated complete differentiation of myoblasts into myotubes; we also detected the expression of the myogenic regulatory factors myoD, myogenin, and desmin. Upregulation (Myogenin, desmin) and downregulation (MyoD) of muscle regulation factors confirmed the differentiation in OFMCs.

Cells, Free Full-Text

Cells, Free Full-Text

Iec/Ts 62886 Get File - Colaboratory

The cell-free system: A new apparatus for affordable, sensitive, and portable healthcare - ScienceDirect

Five-Year Outcomes for Refractory B-Cell Lymphomas with CAR T-Cell Therapy

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Cell-Free DNA and Apoptosis: How Dead Cells Inform About the Living - ScienceDirect

An illustration of the full-duplex cell-free massive MIMO system.

Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods, Microbial Cell Factories

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Labile coat: reason for noninfectious cell-free varicella-zoster virus in culture. - Abstract - Europe PMC

Cell-free methods to produce structurally intact mammalian membrane proteins

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Sequencing of Circulating Cell-free DNA during Pregnancy