Cells, Free Full-Text

High-resolution 3D images of organelles are of paramount importance in cellular biology. Although light microscopy and transmission electron microscopy (TEM) have provided the standard for imaging cellular structures, they cannot provide 3D images. However, recent technological advances such as serial block-face scanning electron microscopy (SBF-SEM) and focused ion beam scanning electron microscopy (FIB-SEM) provide the tools to create 3D images for the ultrastructural analysis of organelles. Here, we describe a standardized protocol using the visualization software, Amira, to quantify organelle morphologies in 3D, thereby providing accurate and reproducible measurements of these cellular substructures. We demonstrate applications of SBF-SEM and Amira to quantify mitochondria and endoplasmic reticulum (ER) structures.

Cells, Free Full-Text

Antibodies, Free Full-Text

HIV-1 cell-to-cell transmission and broadly neutralizing antibodies, Retrovirology

Cells, Free Full-Text

Sequencing of Circulating Cell-free DNA during Pregnancy

Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods, Microbial Cell Factories

Cell-free Macromolecular Synthesis

Ds Kumar Strömungsmechanik Pdf Kostenloser Download - Colaboratory

PDF] Mesenchymal Stem Cell-Derived Exosomes as New Remedy for the Treatment of Neurocognitive Disorders

Five-Year Outcomes for Refractory B-Cell Lymphomas with CAR T-Cell Therapy