Biology, Free Full-Text

In addition to representing a significant part of the natural background radiation exposure, α-particles are thought to be a powerful tool for targeted radiotherapy treatments. Understanding the molecular mechanisms of recognition, signaling, and repair of α-particle-induced DNA damage is not only important in assessing the risk associated with human exposure, but can also potentially help in identifying ways of improving the efficacy of radiation treatment. α-particles (He2+ ions), as well as other types of ionizing radiation, and can cause a wide variety of DNA lesions, including DNA double-strand breaks (DSBs). In mammalian cells, DNA DSBs can be repaired by two major pathways: non-homologous end-joining (NHEJ) and homologous recombination (HR). Here, we investigated their dynamics in mouse NIH-3T3 cells through the recruitment of key proteins, such as the KU heterodimer for NHEJ and RAD51 for HR upon localized α-particle irradiation. To deliver α-particles, we used the MIRCOM microbeam, which allows targeting of subnuclear structures with submicron accuracy. Using mouse NIH-3T3 cells, we found that the KU heterodimer is recruited much earlier at DNA damage sites marked by H2AX phosphorylation than RAD51. We also observed that the difference in the response of the KU complex and RAD51 is not only in terms of time, but also in function of the chromatin nature. The use of a microbeam such as MIRCOM, represents a powerful tool to study more precisely the cellular response to ionizing irradiation in a spatiotemporal fashion at the molecular level.

Genes, Free Full-Text, Mapping DNA Topoisomerase Binding and Cleavage Genome Wide Using Next-Generation Sequ…

A primer on emerging field-deployable synthetic biology tools for global water quality monitoring

ArchAlign: coordinate-free chromatin alignment reveals novel architectures, Genome Biology

Full course on Cd-rom and solutions and tests on Cd-rom. This is cds NO BOOKS INCLUDED.

Apologia Biology (2nd Ed) By Jay Wile Text & Solutions/tests On CD-ROM

Edexcel A level Biology: Yr 1 & 2 Complete Revision and Practice - Cheshire College South & West

AP Biology Premium, 2022-2023: by: Mary Wuerth - 9781506280370

A piece of biology text annotated with multiple ontologies.

Free Curriculum Vitae Example

Skmer: assembly-free and alignment-free sample identification using genome skims, Genome Biology

Cells Penworthy Prebound Books

Biology the easy way : Edwards, Gabrielle I : Free Download, Borrow, and Streaming : Internet Archive

Biology, Free Full-Text

Stream ⚡ Princeton Review AP Biology Premium Prep, 26th Edition: 6 Practice Tests + Complete Conten by Layfieldjunkersfeld

What's a Primary Article? - BIO 245: Conservation Biology - LibGuides at Gustavus Adolphus College

Create a Biology Video Online for Free with Biological Templates