In Spider-Man: Into the Spider-Verse (2018), you can see a comic that is a direct reference to Spider-man's first appearance in print form (Amazing Fantasy #15, released in 1962). : r/MovieDetails

Spider-Man turns 60: Marvel comics superhero debuted in 1962

Spider Man First Appearance Amazing Fantasy 15 Sketch Cover, in John K Snyder III's Commissions Comic Art Gallery Room

Spider-Man as he first appeared in the cover of Amazing Fantasy (1962) #15. : r/HeroForgeMinis

Art] Alex Ross pays tribute to Spider-Man's first appearance — Major Spoilers — Comic Book Reviews, News, Previews, and Podcasts

In the 2016 film Batman v Superman Dawn of Justice, as Batman carries Superman, he walks past graffiti that reads “Who watches the watchmen?” which is a nod to Snyder's Watchmen movie. : r/MovieDetails

Spider-Man turns 60: Marvel comics superhero debuted in 1962

AMAZING FANTASY#15 CGC 1.0 FIRST APPEARANCE OF SPIDER-MAN, 8/1962, STAN LEE

Amazing Fantasy 15 Spiderman First Appearance 3d Wooden

In Batman vs Superman(2016) Lex Luthors prisoner number is AC192340. This is a reference to Action Comics #23 (published 1940) the first comic that Lex Luthor makes an appearance. : r/MovieDetails